Talk · AIP conference proceedings · January 1, 2011
Influence of Waveform on Cell Viability during Ultrasound Exposure
Timur M. Saliev, Loreto B. Feril, Donald A. McLean, Katsuro Tachibana, Paul A. Campbell, Yoichiro Matsumoto
Abstract
We examined the role of ultrasound standing waves, and their travelling wave counterparts, on cell viability in an in-vitro insonation apparatus. Furthermore, the effect of distinct waveforms (sine and top-hat) was also explored, together with the role of microbubble presence. Measurements of cell viability in standing wave scenarios demonstrated a relatively higher rate of lysis (63.13 +/- 10.89% remaining viable) compared with the travelling wave data, where 96.22 +/- 4.0% remained viable. Significant differences were also seen as a function of waveform, where insonations employing top-hat wave shapes resulted in an average end stage viability of 30.31 +/- 5.71 % compared with 61.94 +/- 14.28% in the sinusoidal counterparts.
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